HPLC Analyzers

The Tosoh Glycated Hemoglobin Analyzers HLC-723 series allow rapid HbA1c analysis with best-in-class variant detection and high quality β-Thalassemia Screening.

Tosoh’s advanced HPLC systems are fully automated systems that rapidly and precisely separate hemoglobins found naturally in human blood. Suitable for high throughput HbA_1c testing in the laboratory or clinical environment in addition to rapid Hemoglobinopathy screening, our HPLC solutions provide a reliable, accurate and precise service at all times.

Cation Exchange HPLC Principles

HPLC, short for High Performance Liquid Chromatography, is a form of column chromatography (laboratory technique used to separate mixtures) used frequently in biochemistry and analytical chemistry. It involves passing a mixture which contains the &”analyte” through a column (stationary phase), by a liquid (mobile phase) at high pressure.

Cation exchange chromatography is a process that allows the separation of the mixture based on the charge properties of the molecules in the mixture. Cation exchange chromatography retains analyte molecules based on coulombic (ionic) interactions. The stationary phase surface displays negatively charged functional groups that interact with positively charged cations in the mixture.

Tosoh HLC-723 Series Analyzers Separation Principle

The HLC-723G8 and HLC-723GX and HLC-723G11 are fully automated high performance liquid chromatography instrument-reagent systems that rapidly and precisely separate hemoglobins found naturally in blood. Charged hemoglobins and other hemoglobin components are eluted at varying times depending upon the net charge of the molecule in relation to a gradient of increasing ionic strength passed through a non-porous cation exchange column (negatively charged beads).